Process Flow

  • One-stop Service

    One-stop service includes cell pool screening, high yield cell selection, cell amplification, stability prediction, and delivery of cell banks and relevant files. 

  • Customer Cloud Service

    The customer cloud service remotely provides customers with real-time image scanning and image analysis, can locate the target cells in a short time.

    COOPERATION METHOD

  • Pay after Service

    Qualified customers can pay after service without any risks.

Q. How long does it take for Klone4.0 to develop the monoclonal cell line?

 

A:If customers provide cell pool, it takes1.5 months;

      If customers provide sequence, 2.5 months is needed.

 

 

 

Q. Is the monoclonal yield of Klone4.0 higher than that of traditional methods?

 

A:Compared with traditional methods, Klone4.0 can screen far more cells. Therefore, usually K4 can abtain higher yield cells if it stars with the same cell pool.

 

 

 


Q. Will the cells displace during the high-throughput photographing?

 
A:It has been proved that the cell positions can remain traceable, which can meet the experiment requirements.

 

 

 

Q. Will selecting technologies damage the cells?

 

A:Experiments have showed that the technologies won’t damage any cells and the monoclonal formation rate is equivalent to that of traditional technologies.

 

 

 

Q. How to determine monoclonality?

 

A:Klone4.0 platform uses high-throughput photographing technologies to verify the monoclonality.

 

 

 

Q. Does Klone 4.0 have independent intellectual property rights?

 

A:The key technologies involved are all independently developed by GBB. We have a sound intellectual property matrix and IP layouts at domestic and overseas.

 

 

 

Q. How to authorize the vector cell line?

 

A:Cell lines provided by customers needn’t be authorized any more. If customers prefer to use the contracted cell lines of Klone4.0,

      the license fee shall be calculated separately.

Frequently Asked Questions

Platform Introduction

Developed by Great Bay Bio, Klone4.0 is a biotechnological service platform focusing on cell line development. It can screen hundreds of thousands of cells at one time, obtain cell images, and quickly analyze and locate high-yield cells through AI technology and unique cell selecting technology. It can obtain high-yield and high-quality single cells in 4 hours. 

Save up to 80 % workload

 

Develop cell lines in 1.5~2 months

Fast

More

More cell line candidates

Monoclone cells selection in 4 hours

Platform value

High Yield

Much higher Qp than the traditional way

 

Obtain high yield single cells in a short time

Stability Prediction

Original technology

 

Predict the cell line stability in 15 days

Klone4.0™智能化细胞株开发平台

 

 

       Klone4.0™为大湾生物自主研发细胞株开发平台,一次性筛选几十万个细胞,获取细胞影像,通过AI与精准细胞挑选技术,快速分析、定位、选取高产率细胞,数小时内获得高产高质量的细胞株,与细胞株稳定性预测相结合,实现细胞株构建的智能化应用。

 

 

       Klone4.0™将传统细胞株构建的盲目筛选过程转变为主动的精准挑选过程,解决了大量的重复性劳动难题,大幅提高筛选的成功率,具有“快速、低价、高产率”等特点,让细胞株开发更简单更高效。 

自动影像技术

Klone4.0™平台应用细胞影像技术,短期内即可获取几十万个细胞影像,为挑选高产率细胞提供庞大的基数。

数字定位技术

机器深度学习从原始未处理数据集中提取关键特征,使用复杂的分层人工神经网络,让精准定位高产率细胞变成现实。

精准选取技术

Klone4.0™平台运用自主单细胞挑选系统,智能化锁定高产率细胞,并自动、精准挑选单克隆细胞。

技 术 应 用

7.   平台载体细胞株的授权方式 

 

答:a.使用客户提供的细胞株,无需另行授权;
       b.使用平台来源清晰的签约细胞株,授权费用另行计算。

 

6.   Klone4.0™是否具有自主知识产权?

 

答:本平台所涉及的关键步骤均为自主研发,已有完善的知识产权矩阵,在国内外均有IP布局。

5.   如何确定单克隆性?

 

答:Klone4.0™平台采用高通量影像技术,确保所选细胞的单克隆性。

4.   高通量细胞影像拍摄过程中,细胞是否会移位?

 

答:经验证,在实验过程中,细胞位置相对稳定,可满足细胞精准挑选要求。

1.   Klone4.0™单克隆细胞株开发周期

 

答:a. 客户提供细胞池,约1.5个月;

       b. 客户提供序列,约2.5个月。    

3.   细胞挑选技术是否会对细胞造成影响或损伤?

 

答:实验证明,精准挑选技术不会对细胞造成影响或损伤,单克隆形成率与传统筛选技术相当。

2.   与传统方法相比,Klone4.0™平台的单克隆产率是否更高?

 

答:Klone4.0™平台的细胞筛选基数通常比传统方法高出百倍,获得高产率单克隆的概率也大大提高,这是平台的主要优势之一。

      具体产率将由细胞株种类、产品种类、后续操作方式等多种因素决定。

平 台 简 介

平 台 价 值

常 见 问 答